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Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site
C E Peña1, J E Stoner, G F Hatfull
1Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA.
Journal of Bacteriology
|September 1, 1996
Abstract:
Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNAGly gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.