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BTF3 is evolutionarily conserved in fission yeast

J Potashkin1, K Wentz-Hunter, J Callaci

  • 1Department of Pharmacology and Molecular Biology, Finch University of Health Sciences/The Chicago Medical School, IL 60064, USA. potashkj@mis.finchcms.edu

Biochimica Et Biophysica Acta
|September 11, 1996
PubMed
Summary

Researchers identified a fission yeast homolog of the BTF3 protein, spBTF3, which may play a role in transcription initiation. This discovery expands our understanding of BTF3

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Area of Science:

  • Molecular Biology
  • Yeast Genetics

Background:

  • BTF3 protein's potential role in transcription initiation is unclear.
  • BTF3 forms complexes with RNA polymerase II and binds nascent polypeptide chains.

Purpose of the Study:

  • To clone and characterize a homolog of BTF3 from fission yeast, Schizosaccharomyces pombe.

Main Methods:

  • Cloning of the spBTF3 gene from Schizosaccharomyces pombe.
  • Sequence analysis and comparison with known BTF3 homologs.

Main Results:

  • Successfully cloned spBTF3, encoding a 151 amino acid protein.
  • spBTF3 shares high similarity with human and C. elegans BTF3 homologs.
  • Moderate similarity was found with Saccharomyces cerevisiae homologs EGD1 and BTT1.

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Conclusions:

  • The identified spBTF3 homolog provides a new model for studying BTF3 function in transcription.
  • Conserved structural similarities suggest a conserved biological role for BTF3 across species.