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Related Experiment Videos

Surface display of proteins on bacteriophage lambda heads

Y G Mikawa1, I N Maruyama, S Brenner

  • 1Department of Cell Biology, Scripps Research Institute, La Jolla CA 92037, USA.

Journal of Molecular Biology
|September 13, 1996
PubMed
Summary

Researchers created new bacteriophage lambda display vectors. These vectors enable the surface display of foreign proteins fused to the major head protein (gpD), allowing for functional protein expression on phage particles.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Virology

Background:

  • Bacteriophage lambda is a virus that infects bacteria.
  • The major head protein (gpD) of bacteriophage lambda is responsible for forming the capsid.
  • Displaying foreign proteins on phage surfaces can be useful for various applications.

Purpose of the Study:

  • To develop novel plasmid and phage vectors for displaying foreign proteins on the bacteriophage lambda capsid.
  • To enable the fusion of foreign proteins to the major head protein (gpD) of bacteriophage lambda.
  • To ensure the displayed proteins are functionally active and accessible on the phage surface.

Main Methods:

  • Modification of the D gene encoding the major head protein (gpD).
  • Development of vectors with multiple cloning sites, color selection, and conditional chain termination.

Related Experiment Videos

  • Fusion of foreign protein genes to gpD via peptide linkers at N or C termini.
  • Utilizing a host Escherichia coli suppressor activity for controlled assembly.

    • Main Results:

    • Successfully displayed functional beta-lactamase, IgG-binding domains of Staphylococcus aureus protein A, and beta-galactosidase.
    • Constructs expressed active fusion proteins that assembled into phage particles.
    • Demonstrated that gpD can be fused to various peptides and proteins at either terminus.

    Conclusions:

    • The developed vectors are effective for displaying functional foreign proteins on bacteriophage lambda particles.
    • The gpD fusion system offers a versatile platform for protein display and engineering.
    • This technology has potential applications in areas such as antibody discovery and diagnostics.