Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A short CIC-2 mRNA transcript is produced by exon skipping

S Chu1, C B Murray, M M Liu

  • 1Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287-2533, USA.

Nucleic Acids Research
|September 1, 1996
PubMed
Summary

A newly identified short form of the CIC-2 chloride channel (CIC-2S) is transcribed in rat tissues, with lower ratios in the lung. Its genomic organization is conserved between rats and humans.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lattice Radiation Therapy Delays Progression and Extends Survival for Dogs with Macroscopic Soft Tissue Sarcoma.

Radiation research·2026
Same author

[Derivation of occupational exposure limits for propylene glycol monomethyl ether based on mathematical models and uncertainty factors].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2026
Same author

[Visualization analysis of literature on sudden poisoning incidents in China from 2013 to 2022 based on CiteSpace and VOSviewer].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2024
Same author

[Analysis of pollution status and influencing factors of polybrominated diphenyl ethers in household dust in five cities in northern China].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024
Same author

Chromosomal analysis in pregnant women of advanced maternal age: indications for prenatal diagnosis.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2024
Same author

[Discussion on the application status and method improvement of gas chromatography in occupational health inspection standards in China].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2024

Area of Science:

  • Molecular biology
  • Physiology
  • Genomics

Background:

  • CIC-2 is a chloride channel crucial for various tissue functions.
  • Previous research indicated CIC-2 down-regulation in rat lung airways post-birth.
  • Alternative splicing of CIC-2 transcripts was observed during PCR amplification.

Purpose of the Study:

  • To investigate the alternative splicing of CIC-2 transcripts.
  • To characterize the genomic organization of the alternatively spliced region in rats and humans.
  • To determine the tissue-specific expression of CIC-2 isoforms.

Main Methods:

  • Polymerase Chain Reaction (PCR) amplification of rat lung cDNA.
  • Rat and human genomic DNA sequencing.
  • Reverse Transcription PCR (RT-PCR) analysis.

Related Experiment Videos

  • Bioinformatic analysis of DNA sequences.

    • Main Results:

    • A 60 bp shorter CIC-2 transcript (CIC-2S) was identified, originating from an intact exon.
    • Genomic analysis revealed conserved intron-exon structure and splice sites in rats and humans, suggesting alternative splicing.
    • Tissue-specific expression analysis showed varying ratios of long and short CIC-2 transcripts, with the lung exhibiting the lowest long/short ratio.

    Conclusions:

    • The alternatively spliced short form (CIC-2S) is transcribed in multiple rat tissues.
    • Lung tissue has a lower ratio of long to short CIC-2 transcripts compared to brain tissue.
    • The genomic organization facilitating CIC-2 alternative splicing is conserved across rat and human species.