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Role for CD30 in HIV expression
S Romagnani1, F Annunziato, R Manetti
1Institute of Internal Medicine and Immunoallergology, University of Florence, Italy.
Immunology Letters
|June 1, 1996
Summary
Human immunodeficiency virus (HIV) infection increases CD30 expression on T cells, leading to cell death. CD30 signaling also enhances HIV replication, suggesting a role in disease progression.
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- CD30 is a receptor on immune cells, with its ligand CD30L found on B cells, macrophages, and T cells.
- Human immunodeficiency virus (HIV) infects CD4+ T cells, leading to immune deficiency.
Purpose of the Study:
- To investigate the role of CD30 and its ligand CD30L in HIV infection.
- To determine if CD30 signaling affects HIV replication and T-cell survival.
Main Methods:
- In vitro infection of CD4+ T-cell clones with HIV.
- Cross-linking CD30 with monoclonal antibodies.
- Assessing HIV replication in T-cell lines and patient lymph node cells.
- Using anti-CD30L antibodies to inhibit CD30L-mediated effects.
Main Results:
- HIV infection enhanced CD30 expression on CD4+ T cells, preceding cell death.
- CD30 cross-linking potentiated HIV replication in T-cell lines.
- CD30L-expressing CD8+ T cells enhanced HIV replication in CD4+ T cells, an effect blocked by anti-CD30L antibodies.
- Anti-CD30L antibodies suppressed spontaneous HIV replication in patient lymph node cells.
Conclusions:
- CD30 triggering plays a significant role in enhancing HIV replication.
- CD30 signaling may contribute to the death of HIV-infected CD4+ T cells.
- Targeting the CD30 pathway could be a potential therapeutic strategy for HIV.