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Updated: Sep 5, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
Enzymatic method for measuring subnanomolar concentrations of oxygen in gaseous or aqueous solutions
J V O'Fallon1, R W Wright, R E Calza
1Department of Animal Sciences, Washington State University, Pullman, Washington, 99164-6320, USA.
Abstract:
A method that combines glucose oxidase with [U-14C]glucose to measure subnanomolar to decamillimolar concentrations of oxygen is presented. The method is inexpensive and easy to use. It is independent of sample pH and enzyme inhibitors, including detergents and metal ions. This method can be directly applied to gaseous or aqueous samples and multiple assays can be run simultaneously.
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