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Liver preservation below 0 degrees C with UW solution and 2,3-butanediol
M Scotte1, P Eschwege, C Cherruau
1Laboratoire de Recherche Chirurgicale, Faculté de Médecine Cochin-Port Royal, Hôpital Cochin, 27, rue du Faubourg St Jacques, Paris, 75014, France.
Cryobiology
|February 1, 1996
Summary
Preserving rat livers below 0°C with 2,3-butanediol shows promise for extending organ viability. While feasible, potential toxicity of the cryoprotective agent requires further investigation in liver transplantation models.
Area of Science:
- Organ preservation
- Transplantation research
- Cryobiology
Background:
- Long-term liver preservation is crucial for advancing liver transplantation from emergency to elective procedures.
- Current preservation methods limit organ viability, impacting transplant outcomes.
Purpose of the Study:
- To investigate extending cold ischemia time for rat livers.
- To evaluate sub-zero preservation temperatures with a cryoprotective agent.
Main Methods:
- Rat livers were preserved for 72 hours using University of Wisconsin (UW) solution.
- Experimental groups included UW solution with 8% 2,3-butanediol at +4°C or -4°C.
- Liver viability was assessed using an isolated perfused liver model.
Main Results:
- No significant differences in enzymatic release, bile production, or portal venous flow between standard preservation (+4°C) and sub-zero preservation (-4°C).
- Higher enzymatic release was observed in groups preserved with 2,3-butanediol at +4°C compared to standard preservation.
- Sub-zero preservation (-4°C) with 2,3-butanediol appeared feasible, with comparable viability metrics to standard preservation.
Conclusions:
- Preservation of rat livers below 0°C using 2,3-butanediol is feasible.
- Potential toxicity of 2,3-butanediol may counteract benefits of reduced enzymatic activity.
- Further studies using liver transplantation models are needed to confirm the clinical advantages.