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Updated: Aug 19, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Site-Directed Mutagenesis in Epitope Mapping

Benjamin1, Perdue

  • 1Beirne B. Carter Center for Immunology Research and The Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, Virginia, 22908

Methods (San Diego, Calif.)
|June 1, 1996
PubMed

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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