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A procedure for the large-scale isolation of major bovine seminal plasma proteins
J J Calvete1, P F Varela, L Sanz
1Institut für Reproduktionsmedizin, Tierärztliche Hochschule Hannover, Bünteweg 15, Hannover, 30559, Germany.
Protein Expression and Purification
|August 1, 1996
Abstract:
A method for the stepwise purification of bovine seminal plasma proteins aSFP, PDC-109, TIMP-2, RNAse, and BSP-30K/PDC-109 complex is presented. It is based on the use of nondenaturing, classical chromatographic procedures. The isolation protocol is reproducible, inexpensive, easy to carry out in a common laboratory, and yields large amounts of pure proteins for biochemical and structural studies.