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Molecular and Supramolecular Architecture of the Salmo gairdneri Proteinaceous Eggshell during Development
Papadopoulou1, Galanopoulos, Hamodrakas
1Department of Biochemistry, Cell and Molecular Biology, and Genetics, University of Athens, Athens, 15701, Greece
Abstract:
A detailed developmental study of eggshell architecture of the fish Salmo gairdneri (rainbow trout) was performed using transmission and scanning electron microscopy. Thioglycollic acid treatment and freeze-fracturing reveal that fibrils ca. 5-10 nm in diameter constitute each lamella of the helicoidal eggshell. Freeze-fracturing also permits a direct visualization of the helicoidal architecture. Laser-Raman studies of the eggshell indicate abundant antiparallel beta-pleated-sheet conformation in the eggshell proteins of S. gairdneri during all developmental stages. Apparently, this conformation dictates formation of the helicoidal structure. Disulfide bonds, together with isopeptide bonds, cross-link S. gairdneri eggshell proteins throughout development.
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