Sequence of a Giardia lamblia gene coding for the glycolytic enzyme, pyruvate,phosphate dikinase

The sequence of the gene coding for pyruvate,phosphate dikinase (EC 2.7.9.1) in Giardia lamblia (syn. G. duodenalis) has been established. The deduced amino acid sequence is very similar to all of its known homologs from the protist, Entamoeba histolytica, the eubacterium, Clostridium symbiosum and plant chloroplasts. Phylogenetic reconstruction with neighbor-joining and maximum parsimony methods reveals that the sequences form two clades, one comprising the anaerobic microorganisms, and the other the chloroplast enzymes.