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One-day detection of PCR amplified Chlamydia trachomatis DNA in clinical samples: ELISA versus Southern blot
R T Roymans1, G Onland, B H Postma
1Laboratory of Medical Microbiology, AA Veldhoven, The Netherlands.
Aims:
To compare ELISA and Southern blot hybridisation for the detection of PCR amplified Chlamydia trachomatis DNA extracted from clinical samples; to assess the value of the ELISA method in a clinical setting.
Methods:
DNA was extracted from urogenital samples of 508 patients, purified and amplified using C trachomatis specific primers, one of which was endlabelled with biotin. Amplification products were detected by Streptavidin biotin based ELISA and non-radioactive Southern blotting.
Results:
Of the 508 samples, 29 were positive and 479 negative by both methods. No discrepant results were observed.
Conclusion:
Streptavidin biotin based ELISA and Southern blotting were equally sensitive for detecting PCR amplified C trachomatis DNA. Using ELISA, test results could be generated within a single day.