Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Rhodopsin in immature rod outer segments

J Dodge¹, A B Fulton, C Parker

¹Department of Biology, Florida State University, Tallahassee, USA.

Investigative Ophthalmology & Visual Science

|September 1, 1996

Summary

Rhodopsin concentration is low in immature rat rod outer segments (ROS) due to lower opsin amounts and increased disk spacing. These findings suggest a developmental process for rhodopsin accumulation in the retina.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advanced and metastatic prostate cancer: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2026

Same author

Local and locoregional prostate cancer: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2025

Same author

Stereotactic Body Radiotherapy for Oligoprogression in Castration-Resistant Prostate Cancer: Early Toxicity Analysis of the TRAP Trial.

Clinical oncology (Royal College of Radiologists (Great Britain))·2024

Same author

HISTOMONAS MELEAGRIDIS INFECTIONS IN TURKEYS IN THE USA: A CENTURY OF PROGRESS, RESURGENCE, AND TRIBUTE TO ITS EARLY INVESTIGATORS, THEOBALD SMITH, ERNST TYZZER, AND EVERETT LUND.

The Journal of parasitology·2024

Same author

The STAMPEDE2 Trial: a Site Survey of Current Patterns of Care, Access to Imaging and Treatment of Metastatic Prostate Cancer.

Clinical oncology (Royal College of Radiologists (Great Britain))·2023

Same author

Effects of 24-week prebiotic intervention on self-reported upper respiratory symptoms, gastrointestinal symptoms, and markers of immunity in elite rugby union players.

European journal of sport science·2023

Area of Science:

Retinal biology
Photoreceptor development
Molecular ophthalmology

Background:

Rhodopsin is the primary photopigment in rod photoreceptors.
Understanding rhodopsin accumulation is crucial for photoreceptor function.
Rod outer segments (ROS) undergo significant structural and molecular changes during development.

Purpose of the Study:

To investigate rhodopsin concentration in immature rat rod outer segments (ROS).
To test the hypothesis that rhodopsin concentration is low in developing rat ROS.
To explore the relationship between opsin levels and rhodopsin accumulation during rat retinal development.

Main Methods:

Microspectrophotometry (MSP) was used to measure rhodopsin absorbance in localized regions of isolated ROS.

Related Experiment Videos

Rhodopsin and photopigment were extracted from retinas of rats at various developmental stages (13, 19, 34 days, and adult).

The effect of 9-cis retinal on photopigment absorbance was assessed in dark- and light-adapted retinas.

Main Results:

Rhodopsin absorbance was significantly lower in the ROS of immature (13-day-old) rats compared to adults.
In 19-day-old rats, rhodopsin absorbance was low at the ROS tip but adult-level at the base.
9-cis retinal addition increased absorbance in light-adapted retinas across all ages, but not in dark-adapted retinas.

Conclusions:

Rhodopsin accumulation in developing rat rods is a localized, region-dependent process.
Immature rat rods exhibit low rhodopsin absorbance, suggesting reduced opsin levels.
Increased disk-to-disk spacing in immature ROS may contribute to lower rhodopsin concentrations.