Related Experiment Videos
Advanced computer control of electrophysiological experimentation
L Thomsen1, G T Pearson, E Skadhauge
1Institute of Anatomy and Physiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Journal of Neuroscience Methods
|March 1, 1996
Summary
A new Spike2 software configuration offers interactive computer control for intracellular electrophysiology experiments. This system enables precise control over nerve stimulation, current injection, and data acquisition for neuronal analysis.
Area of Science:
- Neuroscience
- Computational Biology
- Physiology
Background:
- Intracellular recording requires precise control over experimental parameters.
- Existing systems may lack flexibility for complex electrophysiological studies.
- Computer-assisted control can enhance data acquisition and analysis efficiency.
Purpose of the Study:
- To develop a computer-controlled system for intracellular electrophysiology.
- To enable interactive control of current injection and nerve stimulation.
- To facilitate the analysis of neuronal properties using custom software.
Main Methods:
- Utilized Spike2 (CED) data acquisition software with a custom configuration.
- Employed the 1401plus computer (CED) as an interface for electrophysiological apparatus.
- Developed a Spike2 programming language analysis tool for neuronal properties.
Main Results:
- Achieved interactive keyboard control of intracellular current injection and nerve stimulation.
- Enabled precise control over pressure ejection and continuous data acquisition.
- Successfully analyzed resting membrane properties, spike characteristics, and synaptic input profiles of enteric neurons.
Conclusions:
- The developed system provides a flexible and controllable platform for intracellular electrophysiology.
- This computer-based setup enhances experimental capabilities for neurophysiologists.
- The hardware and software configurations are valuable for researchers expanding their experimental systems.