[Subfractionation of serum high density lipoproteins and its clinical significance]

Serum concentrations of HDL subfractions, LpAI and LpAI:AII, were simultaneously determined simply, specifically and rapidly by use of rocket immunoelectrophoresis with the home-made gel plates. The method was reproducible, and the results correlated well with those obtained with Sebia's kits. The normal levels of LpAI were 57 +/- 13 mg/dl in males and 59 +/- 15 mg/dl in females. LpAI levels were significantly lower in patients with renal failure and atherosclerosis, and tended to decrease in hepatic diseases than those in normal subjects. The levels of LpAI:AII were also decreased in these three diseased groups. Decreases in LpAI levels were more slightly in patients with hepatic or renal diseases than those in LpAI:AII levels. On the contrary, decrease in LpAI level was more markedly in patients with atherosclerosis than that in the other subfraction. The results in the present study suggest that the determination of HDL subfractions will be useful for diagnosis of various diseases.