Related Experiment Videos
Agarose gel analysis of 15-40-kb PCR amplimers
V Burland1, F P Curtis, N Kusukawa
1FMC BioProducts, Rockland, ME, USA. valerie_burland@fmc.com
Biotechniques
|July 1, 1996
Abstract:
Accurate resolution of PCR products in the range of 15-40 kb may be obtained in agarose gels without pulsed field electrophoresis. A gel of 0.3% SeaKem Gold agarose cast on GelBond support film provides good resolution and sufficient get strength to reliably allow staining and photography. This paper describes a test system for Long PCR and demonstrates analysis of the PCR products on a gel run under standard low-voltage electrophoresis conditions.