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Related Experiment Videos

PSA-NCAM is required for activity-induced synaptic plasticity

D Muller1, C Wang, G Skibo

  • 1Department of Morphology Centre Médical Universitaire, Geneva, Switzerland.

Neuron
|September 1, 1996
PubMed
Summary
This summary is machine-generated.

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Polysialic acid-neural cell adhesion molecule (PSA-NCAM) is crucial for synaptic plasticity. Neuronal activity regulates PSA-NCAM expression, which is essential for long-term potentiation and depression in the hippocampus.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Organotypic hippocampal slice cultures express high levels of polysialylated neural cell adhesion molecule (PSA-NCAM).
  • PSA-NCAM is the embryonic form of neural cell adhesion molecule, crucial for neural development and plasticity.

Purpose of the Study:

  • To investigate the role of PSA-NCAM in synaptic plasticity, specifically long-term potentiation (LTP) and long-term depression (LTD).
  • To determine if neuronal activity regulates PSA-NCAM expression at the synapse.

Main Methods:

  • Treatment of hippocampal slice cultures with endoneuraminidase-N to remove PSA from NCAM.
  • Analysis of LTP and LTD induction in treated slices and in slices from NCAM-deficient transgenic mice.
  • Assessment of NMDA receptor-dependent synaptic responses.

Related Experiment Videos

  • Monitoring of PSA reexpression and recovery of synaptic plasticity.

    • Main Results:

    • Removal of PSA from NCAM completely prevented LTP and LTD induction without affecting cellular or synaptic parameters.
    • Slices from NCAM-deficient mice showed impaired LTP.
    • NMDA receptor-dependent synaptic responses remained unaffected.
    • Washout of the enzyme led to PSA reexpression and recovery of LTP and LTD, regulated by neuronal activity.

    Conclusions:

    • Neuronal activity regulates the synaptic expression of PSA-NCAM.
    • PSA-NCAM expression is essential for the induction of synaptic plasticity, including LTP and LTD.
    • Targeting PSA-NCAM may offer therapeutic strategies for conditions involving impaired synaptic plasticity.