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Related Experiment Videos

Electromagnetic field induced changes in lipid second messengers

S Clejan1, C Ide, C Walker

  • 1Department of Pathology, Tulane University School of Medicine, New Orleans, LA 70112-2699, USA. clejan@tmc.tulane.edu

Journal of Lipid Mediators and Cell Signalling
|May 1, 1996
PubMed
Summary
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Pulsed magnetic fields exhibit anti-proliferative effects on TF-1 cells by altering the cell cycle. These fields also modulate lipid signal transduction pathways, specifically up-regulating Phospholipase D (PLD) while potentially inactivating Phospholipase C (PLC).

Area of Science:

  • Cell Biology
  • Biophysics
  • Signal Transduction

Background:

  • Electromagnetic fields (EMFs) have shown varied effects on cellular processes.
  • Lipid signal transduction is crucial for cell cycle regulation.
  • Human hematopoietic cell line TF-1 is a model for studying cellular responses.

Purpose of the Study:

  • To investigate the impact of pulsed magnetic fields (PMFs) on the cell cycle of TF-1 cells.
  • To analyze the effects of PMFs on key lipid second messengers, diacylglycerol (DAG) and phosphatidic acid (PA).
  • To elucidate the involvement of phospholipase D (PLD) and phospholipase C (PLC) pathways in cellular response to PMFs.

Main Methods:

  • Flow cytometry was used to assess cell cycle distribution (G1 and S phases).
  • Measurement of DAG and PA levels following PMF exposure.

Related Experiment Videos

  • Assessment of Phospholipase D (PLD) activity via transphosphatidylation assays.
  • Kinetic analysis of [3H]choline and [3H]phosphocholine formation.
  • Inhibition studies using propranolol, a PA phosphohydrolase inhibitor.

    • Main Results:

    • PMF exposure (30-40 pulses) resulted in a 31% increase in G1 phase and a 35% decrease in S phase, indicating anti-proliferative effects.
    • PMF stimuli significantly altered DAG and PA levels in a pulse-dependent manner.
    • PLD pathway was upregulated, evidenced by increased [3H]Phosphatidylethanol formation and transphosphatidylation.
    • Evidence suggests temporary inactivation of the PC-PLC pathway and upregulation of the PC-PLD pathway.

    Conclusions:

    • Pulsed magnetic fields exert anti-proliferative effects on TF-1 cells.
    • PMFs modulate lipid second messenger dynamics, implicating PLD and PLC pathways.
    • The findings provide insights into the molecular mechanisms underlying cellular responses to EMFs.