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Updated: Aug 8, 2026
Dissection of Human Vitreous Body Elements for Proteomic Analysis
Published on: January 24, 2011
Perioperative analysis of vitreous cell components by immunoimpression cytology
1Augenklinik, Liebigstrasse 22b, Leipzig, Germany.
Background:
Perioperative analysis and classification of vitreous cell components needs a quick method sufficient to prepare both single cells and cellular membranes.
Methods:
In an attempt to clarify the usefulness of immunoimpression cytology we examined 82 eyes with proliferative vitreoretinopathy, proliferative diabetic retinopathy, perforating injuries and contusions.
Results:
With this method it is possible to perioperatively obtain cells from surfaces of vitreous membranes as well as single cells in suspension. After staining, various cell types could be differentiated morphologically and on the basis of antibody response to cell markers. We found single cells reacting with the antibodies 27E10 (4 positive of 14 tested), vimentin (2/12), RM 3/1 (2/15), LCA (1/ 11) and, particularly, with antiproliferating cell nuclear antigen (9/18).
Conclusion:
With immunoimpression cytology it is possible to determine cell surface markers rapidly and accurately from specimens obtained at the time of vitrectomy. The clinical utility of this test will be ascertained by future studies.
