Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell migration: interactions among integrins, IGFs and IGFBPs

J I Jones1, M E Doerr, D R Clemmons

  • 1Department of Medicine, University of North Carolina, Chapel Hill 27599-7170, USA.

Progress in Growth Factor Research
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

What has happened to river macroinvertebrate biodiversity in England and Wales over the past 30 years?

Journal of environmental management·2026
Same author

Opportunities, approaches and challenges to the engagement of citizens in filling small water body data gaps.

Hydrobiologia·2022
Same author

Determining the sources of nutrient flux to water in headwater catchments: Examining the speciation balance to inform the targeting of mitigation measures.

The Science of the total environment·2018
Same author

Do submerged aquatic plants influence their periphyton to enhance the growth and reproduction of invertebrate mutualists?

Oecologia·2017
Same author

An anti-αVβ3 antibody inhibits coronary artery atherosclerosis in diabetic pigs.

Atherosclerosis·2017
Same author

Tackling agricultural diffuse pollution: What might uptake of farmer-preferred measures deliver for emissions to water and air?

The Science of the total environment·2016
Same journal

Proceedings of the 3rd International Symposium on IGF Binding Proteins. Tubingen, Germany, 6-8 October 1995.

Progress in growth factor research·1995
Same journal

Components of the IGF system mediate the opposing effects of tamoxifen on endometrial and breast cancer cell growth.

Progress in growth factor research·1995
Same journal

Antiproliferative actions of insulin-like growth factor binding protein (IGFBP)-3 in human breast cancer cells.

Progress in growth factor research·1995
Same journal

Regulation of IGFBP-3 expression in breast cancer cells and uterus by estradiol and antiestrogens: correlations with effects on proliferation: a review.

Progress in growth factor research·1995
Same journal

Insulin-like growth factors (IGF) and IGF binding proteins in children with chronic renal failure.

Progress in growth factor research·1995
Same journal

Developmental regulation of circulating insulin-like growth factor-binding proteins in normal pregnancies and in pre-eclampsia.

Progress in growth factor research·1995
See all related articles

Insulin-like growth factor-binding proteins (IGFBPs) and IGF-I directly influence cell migration by engaging integrin receptors. These findings are crucial for understanding processes like tumor metastasis and wound healing.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Cell migration is vital for numerous biological processes, including tumor metastasis, embryonic development, and wound healing.
  • Insulin-like growth factor-I (IGF-I) and its binding proteins (IGFBPs) are known modulators of cellular functions.

Purpose of the Study:

  • To investigate the effects of IGF-I and IGFBPs on the migration of Chinese Hamster ovary (CHO) cells, smooth muscle cells (SMC), and human breast cancer cells (HBC).
  • To elucidate the role of integrin receptors in IGF-I and IGFBP-induced cell migration.

Main Methods:

  • Monolayer wounding assay to assess SMC and CHO cell migration.
  • Boyden chamber assay to evaluate HBC migration.
  • Analysis of integrin receptor involvement in migration pathways.

Related Experiment Videos

Main Results:

  • IGFBP-1 directly stimulated SMC migration via the alpha 5 beta 1 integrin, similar to its effect on CHO cells.
  • IGF-I stimulated SMC migration, an effect inhibited by IGFBP-1 and IGFBP-2.
  • IGF-I acted as a potent chemoattractant for MCF-7 HBC, requiring specific integrin interactions (alpha V beta 5 or alpha 2 beta 1) for migration on different substrates.

Conclusions:

  • Cell migration stimulated by IGFBP-1 and IGF-I involves signaling through integrin receptors.
  • Specific integrins mediate migration responses to IGF-I and IGFBPs in different cell types.
  • The precise interaction mechanisms between IGF-I receptor and integrin signaling pathways remain to be determined.