Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Different methods for measuring endogenous digitalis-like factor(s)

A Paci1, S Balzan, A Ledda

  • 1Istituto di Fisiologia Clinica C.N.R., Pisa, Italy.

Journal of Pharmaceutical and Biomedical Analysis
|June 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevated mortality risks associated with late diagnosis of cancer in individuals with psychiatric disorders?

Journal of psychiatric research·2024
Same author

Sex differences in the pharmacokinetics of anticancer drugs: a systematic review.

ESMO open·2024
Same author

Stop antibiotics when you feel better? Opportunities, challenges and research directions.

JAC-antimicrobial resistance·2024
Same author

Prognostic value of total, free and lipoprotein fraction-bound plasma mitotane levels in advanced adrenocortical carcinoma: a prospective study of the ENDOCAN-COMETE-Cancer network.

Journal of endocrinological investigation·2024
Same author

Association of cabozantinib pharmacokinetics, progression and toxicity in metastatic renal cell carcinoma patients: results from a pharmacokinetics/pharmacodynamics study.

ESMO open·2021
Same author

Pycnogenol® Supplementation Prevents Recurrent Urinary Tract Infections/Inflammation and Interstitial Cystitis.

Evidence-based complementary and alternative medicine : eCAM·2021
Same journal

Analyzing the impact of ionizable lipid identity, purity, and stability on lipid nanoparticle performance.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Application of two-dimensional liquid chromatography as a complementary technique to circular dichroism spectroscopy and high-resolution mass spectrometry for the characterization of GalNAc-siRNA conjugates.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

The transfer of per- and polyfluoroalkyl substances (PFAS) from mother to child: Comparison between maternal and cord blood in an Italian cohort.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

UHPLC/Q-TOF-MS-based blood-component profiling and multi-omics analysis reveal potential protective mechanisms of Shenzhuo Formula against diabetic kidney disease.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Multi-center study of the recombinant cascade reagent (kinetic chromogenic assay) as an alternative method for bacterial endotoxin testing: Method validation, product suitability, and consistency evaluation with limulus amebocyte lysate.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Simultaneous enantioselective separation of 2-, 3- and 4-chloromethcathinones using supercritical fluid chromatography-tandem mass spectrometry and its application to human oral fluid samples.

Journal of pharmaceutical and biomedical analysis·2026
See all related articles

Accurately measuring endogenous digitalis-like factors (EDLF) is challenging due to a lack of specific methods. This study evaluated radioreceptor assay, 86Rb uptake inhibition, and immunoassay, finding each has limitations for reliable EDLF detection.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Analytical Chemistry

Background:

  • Identifying endogenous digitalis-like factors (EDLF) is crucial for understanding physiological processes.
  • A significant challenge in EDLF research is the absence of a singular, precise measurement technique.
  • Existing methods for EDLF detection vary in specificity and biological relevance.

Purpose of the Study:

  • To evaluate and compare different methods for the accurate measurement of endogenous digitalis-like factors (EDLF).
  • To assess the suitability of radioreceptor assay (RRA), 86Rb uptake inhibition, and ouabain immunoassay for EDLF detection.
  • To determine the limitations and advantages of each assay in identifying biologically active EDLF.

Main Methods:

  • Plasma samples from normal adults and patients were extracted using C18 solid-phase and affinity resin chromatography.

Related Experiment Videos

  • Evaluated methods included human placenta radioreceptor assay (RRA), 86Rb uptake inhibition in erythrocytes, and a specific ouabain enzyme immunoassay (EIA).
  • Assays were compared based on ease of use, biological meaningfulness, specificity, and correlation with purified EDLF.

    • Main Results:

    • Human placenta RRA offers biological relevance but shares ease of use with EIA.
    • Ouabain EIA showed correlation with RRA but sometimes failed to detect EDLF, and its high specificity for ouabain may miss related factors.
    • The 86Rb uptake inhibition method correlated with RRA for HPLC-purified EDLF and reflects in-vivo activity but is impractical for routine clinical use due to sample handling requirements.

    Conclusions:

    • Each assay method for detecting endogenous digitalis-like factors (EDLF) possesses inherent limitations.
    • No single assay is sufficient for comprehensive and accurate EDLF measurement.
    • The study recommends employing multiple assays concurrently for robust EDLF detection and characterization.