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Related Experiment Videos

Novel diffusion cell for in vitro transdermal permeation, compatible with automated dynamic sampling

I J Bosman1, A L Lawant, S R Avegaart

  • 1Groningen Institute for Drug Studies (GIDS), University Centre for Pharmacy, Department of Analytical Chemistry and Toxicology, Netherlands.

Journal of Pharmaceutical and Biomedical Analysis
|June 1, 1996
PubMed
Summary

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A novel Kelder cell system automates in vitro transdermal drug permeation studies. This dynamic system improves upon static Franz diffusion cells by mimicking blood flow and maintaining sink conditions for accurate drug permeation analysis.

Area of Science:

  • Pharmacology
  • Biomedical Engineering
  • Drug Delivery Systems

Background:

  • In vitro transdermal permeation studies are crucial for drug development.
  • Traditional Franz diffusion cells have limitations in maintaining dynamic conditions and automation.
  • There is a need for advanced systems to accurately assess drug permeation through skin models.

Purpose of the Study:

  • To develop and describe a new automated diffusion cell system, the Kelder cell.
  • To evaluate the performance of the Kelder cells for in vitro transdermal permeation studies.
  • To compare the Kelder cell system with the static Franz diffusion cell.

Main Methods:

  • The study utilized Kelder cells integrated with the ASPEC system for automated sampling.
  • The Kelder cells were designed with specific inlet, donor, and receptor compartments to prevent air bubbles.

Related Experiment Videos

  • Experiments involved permeation of atropine through a Silastic membrane, mimicking transdermal drug delivery.

    • Main Results:

    • The Kelder cell system allowed for automatic sampling of up to 20 cells over 24 hours in a dynamic mode.
    • The design of the Kelder cells effectively prevented air bubble entrapment.
    • The system successfully mimicked physiological blood flow by replacing the permeating drug every 2 minutes, enhancing sink conditions.
    • Permeation experiments with atropine demonstrated minimized variability compared to human skin using the artificial membrane.

    Conclusions:

    • The developed Kelder cell system offers an effective automated solution for in vitro transdermal permeation.
    • This dynamic system provides improved control over experimental conditions, such as sink conditions, compared to static cells.
    • The Kelder cells, when used with artificial membranes, reduce variability and enhance the reliability of transdermal drug permeation studies.