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Three different cell types can synthesize factor XIII subunit A in the human liver
1Department of Anatomy, Histology and Embryology, University School of Medicine, Debrecen, Hungary.
Thrombosis and Haemostasis
|July 1, 1996
Summary
Human Factor XIII subunit A (FXIII A) is synthesized in the liver by Kupffer cells, histiocytes, and hepatocytes. This study identifies specific liver cells responsible for FXIII A production, clarifying its extra-hemopoietic origin.
Area of Science:
- Hematology
- Cell Biology
- Hepatology
Background:
- The origin of human Factor XIII subunit A (FXIII A) has been debated, with hemopoietic cells identified as a source, but extra-hemopoietic sites remain unclear.
- The liver is a suspected extra-hemopoietic source of plasma FXIII A, yet the specific synthesizing cells were not identified.
Purpose of the Study:
- To determine the cellular distribution of FXIII A and its encoding mRNA in human liver samples.
- To identify the specific liver cell types responsible for FXIII A synthesis.
Main Methods:
- Utilized light- and electron-microscopic immuno-morphological techniques.
- Employed in situ hybridization to detect FXIII A mRNA.
- Used postembedding immunocytochemical methods for cellular localization.
Main Results:
- FXIII A was detected in Kupffer cells, connective tissue histiocytes, and hepatocytes within the human liver.
- Immunoreactive hepatocytes were predominantly found near the venae centrales.
- FXIII A mRNA was localized to the same cell types (Kupffer cells, histiocytes, hepatocytes).
Conclusions:
- Human liver contains at least three distinct cell types capable of synthesizing FXIII A: Kupffer cells, histiocytes, and hepatocytes.
- Further studies are needed to quantify the contribution of these liver cells to the overall plasma FXIII A levels.