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Related Experiment Videos

Complex interaction between different proteinaceous components within the cell-wall structure of Candida albicans

J L Lopez-Ribot1, D A Cortlandt, D C Straus

  • 1Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.

Mycopathologia
|November 1, 1995
PubMed
Summary

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This study investigates a monoclonal antibody (MAb) DC3:H10 targeting Candida albicans germ tubes. Findings suggest MAb DC3:H10 interacts with C. albicans C3d receptor (CR2) and mannoprotein components.

Area of Science:

  • Mycology
  • Immunology
  • Biochemistry

Background:

  • A previously identified monoclonal antibody, MAb DC3:H10, targets an epitope on Candida albicans germ tubes.
  • Further characterization of the MAb-reactive material is essential for understanding fungal cell wall interactions.

Purpose of the Study:

  • To further examine the material recognized by MAb DC3:H10.
  • To investigate the relationship between the MAb DC3:H10 epitope and other known cell wall components, specifically the C. albicans C3d receptor (CR2) and a fibrinogen binding mannoprotein (mp58).

Main Methods:

  • Immunoblot analysis using partially purified MAb-reactive material.
  • Enzyme-linked immunosorbent assay (ELISA) with MAb DC3:H10 and polyclonal antibodies against CR2 and mp58.
  • Indirect immunofluorescence (IIF) and dual labeling IIF to assess epitope distribution and interactions.

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Main Results:

  • The MAb-reactive material showed reactivity with antibodies to the C. albicans CR2.
  • MAb DC3:H10 captured antigen recognized by anti-CR2 and anti-mp58 antibodies, but did not compete with them, indicating distinct epitopes.
  • IIF revealed differential surface distribution of epitopes, with MAb DC3:H10 binding patterns being influenced by anti-CR2 antibody but not by fibrinogen or anti-mp58 antibody.

Conclusions:

  • The MAb DC3:H10 epitope is associated with C. albicans CR2 and mp58, but represents a distinct antigenic site.
  • A complex interaction exists between the MAb DC3:H10 recognized epitope and the CR2 component on the C. albicans cell surface.