Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The developing avian retina expresses agrin isoforms during synaptogenesis

S Kröger1, S E Horton, L S Honig

  • 1Department of Neuroanatomy, Max-Planck-Institute for Brain Research, Frankfurt, Germany.

Journal of Neurobiology
|February 1, 1996
PubMed
Summary

Agrin, a molecule crucial for organizing synapses, is present in the developing avian retina during synapse formation. Its distribution and expression suggest agrin isoforms play a role in central nervous system synapse development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dominantly Inherited Alzheimer Network Trials Unit (DIAN-TU): Trial Satisfaction and Attitudes towards Future Clinical Trials.

The journal of prevention of Alzheimer's disease..·2024
Same author

Effects of Vascular Risk Factors on the Association of Blood-Based Biomarkers with Alzheimer's Disease.

Medical research archives·2023
Same author

A large-scale study of microplastic abundance in sediment cores from the UK continental shelf and slope.

Marine pollution bulletin·2022
Same author

Quality of cochlear implant rehabilitation under COVID-19 conditions.

HNO·2020
Same author

[Quality of cochlear implant rehabilitation under COVID-19 conditions. German version].

HNO·2020
Same author

An approach for the identification of exemplar sites for scaling up targeted field observations of benthic biogeochemistry in heterogeneous environments.

Biogeochemistry·2020

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Agrin is a key molecule involved in synapse organization.
  • Understanding agrin's role in the developing nervous system is crucial for insights into neural development.

Purpose of the Study:

  • To investigate the localization, isoform pattern, and mRNA distribution of agrin in the developing avian retina.
  • To determine the role of agrin during synapse formation in the central nervous system.

Main Methods:

  • Injection of anti-agrin Fab fragments into chick eyes to label extracellular agrin.
  • Polymerase chain reaction (PCR) analysis to identify agrin isoforms.
  • In situ hybridization to study mRNA distribution of agrin isoforms.

Related Experiment Videos

Main Results:

  • Extracellular agrin staining was observed in the inner and outer plexiform layers, changing from diffuse to punctate during synapse formation.
  • Agrin was intensely labeled in the inner limiting membrane and axonal processes of retinal ganglion cells.
  • Multiple agrin isoforms were expressed in the developing neural retina, with mRNA localized in the ganglion cell and inner nuclear layers.

Conclusions:

  • The presence and distribution of agrin isoforms in the developing avian retina support its role in synapse formation.
  • Agrin likely plays a significant role in the development of synapses within the central nervous system.