Dendrites of locus coeruleus neurons extend preferentially into two pericoerulear zones

The intrinsic cytoarchitecture and neurochemical organization of the nucleus locus coeruleus have been characterized extensively, but there is little information about the organization of locus coeruleus neuronal processes extending outside of the nucleus proper. Light and electron microscopic immunocytochemical techniques were used to investigate the distribution of dopamine-beta-hydroxylase- or tyrosine-hydroxylase-labeled extranuclear processes in the rat pericoerulear region. The vast majority of these processes extended preferentially into two zones: (1) the pontine tegmentum medial and rostral to locus coeruleus, here termed the rostromedial pericoerulear region; and (2) a narrow region adjacent to the IVth ventricle caudomedial to locus coeruleus, designated here as the caudal juxtaependymal pericoerulear region. Far fewer labeled processes extended into the lateral and ventral pericoerulear regions. Seventy-seven percent of the labeled profiles in the pericoerulear region were dendrites. All labeled profiles in the rostromedial pericoerulear region and 94% of the labeled profiles in the caudal juxtaependymal zone were dendrites. By contrast, in the rostroventral pericoerulear region, 25% of the labeled profiles were axons. Locus coeruleus extranuclear dendrites were never presynaptic to other structures but were often contacted by several unlabeled presynaptic terminals. These results indicate that the dendrites of locus coeruleus neurons extend preferentially into two pericoerulear zones. Extranuclear dendrites in all pericoerulear regions receive extensive, nonnoradrenergic synaptic contacts. Thus, pericoerulear dendrites, particularly in the rostromedial and caudal juxtaependymal zones, are important sites for the integration of inputs to locus coeruleus neurons.