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Creatine kinase activity decrease with short-term freezing

E I Lev1, I Hendler, R Siebner

  • 1Medical Corps, Israel Defence Forces.

Enzyme & Protein
|January 1, 1994
PubMed
Summary
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Freezing human serum at -30°C without additives significantly reduces creatine kinase (CK) activity over time. This enzyme instability impacts the accuracy of CK measurements in stored serum samples.

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Laboratory Medicine

Background:

  • Serum banks commonly store samples at -30°C without protective agents for cost-effectiveness.
  • Creatine kinase (CK) is a crucial enzyme marker in clinical diagnostics.
  • Understanding enzyme stability during sample storage is vital for reliable results.

Purpose of the Study:

  • To assess the stability of creatine kinase (CK) activity in human serum stored at -30°C.
  • To quantify the percentage decrease in CK activity after different storage durations.

Main Methods:

  • Human serum samples were stored at -30°C for 24 hours, 2 weeks, and 4 weeks.
  • Creatine kinase activity was measured in fresh samples and compared to matched samples post-storage.
  • Statistical analysis was performed to determine the significance of observed changes.

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Main Results:

  • A significant decrease in median CK activity was observed after 24 hours (2.6%), 2 weeks (5.9%), and 4 weeks (8.3%) of storage.
  • The decline in CK activity was steady and statistically significant across all time points (p < 0.0001).
  • Initial CK levels or sex did not influence the rate of enzyme degradation.

Conclusions:

  • Serum storage at -30°C without cryoprotectants leads to a consistent and significant loss of creatine kinase activity.
  • The findings highlight the need to consider sample storage conditions when interpreting CK levels in frozen serum.
  • These results are important for both clinical diagnostics and research utilizing stored serum samples.