Neurotensin projections to subfornical organ from arcuate nucleus

Two series of experiments were done in the rat to investigate whether neurons in arcuate nucleus of the hypothalamus (Arc) containing neurotensin (NT)-like immunoreactivity projected to subfornical organ (SFO). In the first series, the anterograde tract-tracer Phaseolus vulgaris leucoagglutinin (PHA-L) was microiontrophoresed into the region of Arc that contains NT neurons. After a 9-12 day survival period the animals were sacrificed and forebrain sections that contained SFO were processed for combined PHA-L and NT immunoreactivity. In the second series of experiments, unilateral or bilateral electrolytic lesions of Arc were made and after a 10-17 day survival period SFO was examined to determine the relative contribution of NT Arc neurons to NT immunoreactivity within SFO. PHA-L labelled fibers with terminal-like boutons were found in SFO primarily on the side ipsilateral to the site of injection. A small number of the PHA-L labelled fibers in the lateral aspect of SFO was also immunoreactive to NT. Unilateral lesions reduced, whereas bilateral lesions of Arc eliminated most of the NT-like immunoreactivity within SFO. These data demonstrate the existence of a direct pathway from Arc to SFO that contains the putative neurotransmitter NT. These results suggest that this pathway may function in the modulation of neural and/or humoral events related to cardiovascular regulation and body fluid homeostasis by influencing the activity of SFO neurons.