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Related Experiment Videos

Area postrema voltage-activated calcium currents

M Hay1, E M Hasser, K A Lindsley

  • 1Department of Physiology, University of Texas Health Science Center, San Antonio 78284-7756, USA.

Journal of Neurophysiology
|January 1, 1996
PubMed
Summary
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Rabbit area postrema neurons exhibit distinct low- and high-threshold calcium currents. These currents are modulated by specific ions and peptides, revealing novel channel properties and potential drug targets for neurological conditions.

Area of Science:

  • Neuroscience
  • Cell Physiology

Background:

  • The area postrema plays a crucial role in regulating physiological functions.
  • Understanding calcium channel activity in these neurons is vital for comprehending their function.

Purpose of the Study:

  • To characterize voltage-activated calcium currents in rabbit area postrema neurons.
  • To identify the types of calcium channels present and their pharmacological properties.

Main Methods:

  • Utilized the perforated patch-clamp technique to record calcium currents.
  • Employed specific ions (Ni2+, Cd2+) and toxins (nimodipine, omega-conotoxins) to differentiate channel types.
  • Investigated the effects of Arg-vasopressin on calcium currents.

Main Results:

Related Experiment Videos

  • Identified both low-threshold (T-type) and high-threshold calcium currents.
  • T-type currents were present in 64% of neurons, while high-threshold currents were ubiquitous.
  • Nickel ions inhibited both current types, whereas cadmium primarily affected high-threshold currents.
  • Nimodipine and omega-conotoxin-GVIA were ineffective, ruling out L- and N-type channels.
  • Omega-conotoxin MVIIC significantly inhibited high-threshold currents, indicating a novel channel subtype.
  • Arg-vasopressin selectively enhanced the transient component of high-threshold currents.

    • Conclusions:

    • Rabbit area postrema neurons possess distinct low- and high-threshold calcium currents.
    • A novel omega-conotoxin MVIIC-sensitive high-threshold calcium channel is present.
    • Arg-vasopressin modulates high-threshold calcium currents, suggesting a role in neuronal signaling.