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Effect of phosphate ion on mildiomycin production by Streptoverticillium rimofaciens
K Kishimoto1, Y S Park, M Okabe
1Technology Department Hikari branch Lab., Takeda Chemical Industries, Ltd., Yamaguchi Prefecture, Japan.
Abstract:
Effect of inorganic phosphate and ammonium nitrogen on mildiomycin production by Streptoverticillium rimofaciens was investigated in culture with and without addition of ferrous ion. In the presence of ferrous ion, the suitable supply of inorganic phosphate increased the intracellular ATP, but that was not observed without ferrous ion addition. The intracellular ATP remarkably affected ammonium nitrogen assimilation and mildiomycin production, and its concentration in the ferrous-sufficient culture was about 2 approximately 3 times higher than that in the ferrous-deficient culture. The low concentration of intracellular ATP in the ferrous-deficient culture resulted in the reduction of ammonium nitrogen assimilation and mildiomycin biosynthesis. This phosphate ion effect on the intracellular ATP concentration was demonstrated only when ferrous ion was added into the medium. These suggest that mildiomycin biosynthesis is regulated through the concentration of intracellular ATP related to the ammonium nitrogen assimilation.
Insights
Ferrous ion is crucial for inorganic phosphate to boost intracellular ATP, enhancing mildiomycin production and ammonium nitrogen assimilation in Streptoverticillium rimofaciens.
Area of Science:
- Microbiology
- Biochemistry
- Fermentation Technology
Background:
- Mildiomycin is an important antibiotic produced by Streptoverticillium rimofaciens.
- Understanding the regulation of mildiomycin biosynthesis is crucial for optimizing its production.
- Intracellular energy status, particularly ATP levels, plays a significant role in microbial secondary metabolism.
Purpose of the Study:
- To investigate the effects of inorganic phosphate and ammonium nitrogen on mildiomycin production.
- To elucidate the role of ferrous ion in modulating these effects.
- To understand the relationship between intracellular ATP, nutrient assimilation, and mildiomycin biosynthesis.
Main Methods:
- Cultivation of Streptoverticillium rimofaciens under varying conditions of inorganic phosphate, ammonium nitrogen, and ferrous ion.
- Measurement of intracellular ATP concentrations.
- Quantification of mildiomycin production.
- Assessment of ammonium nitrogen assimilation.
Main Results:
- Inorganic phosphate increased intracellular ATP in the presence of ferrous ion, but not in its absence.
- Intracellular ATP levels significantly influenced ammonium nitrogen assimilation and mildiomycin production.
- Ferrous-sufficient cultures showed 2-3 times higher intracellular ATP than ferrous-deficient cultures, correlating with higher mildiomycin yields.
- Low intracellular ATP in ferrous-deficient cultures led to reduced ammonium assimilation and mildiomycin biosynthesis.
Conclusions:
- Mildiomycin biosynthesis is regulated by intracellular ATP concentration.
- Ferrous ion is essential for inorganic phosphate to enhance intracellular ATP levels.
- The interplay between ferrous ion, inorganic phosphate, and intracellular ATP is critical for efficient mildiomycin production and nutrient assimilation.