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Structural characterization of red wine rhamnogalacturonan II

P Pellerin1, T Doco, S Vidal

  • 1Institut National de la Recherche Agronomique, Laboratoire des Polymères et des Techniques Physico-Chimiques, Montpellier, France.

Carbohydrate Research
|September 2, 1996
PubMed
Summary

Red wine contains rhamnogalacturonan II (RG-II), a complex pectic polysaccharide. Studies reveal its structure is conserved with plant cell walls, with some variations in dimerization and esterification.

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Area of Science:

  • Biochemistry
  • Plant Cell Wall Biology
  • Food Chemistry

Background:

  • Rhamnogalacturonan II (RG-II) is a significant pectic polysaccharide found in plant cell walls and red wine.
  • Understanding RG-II structure is crucial for comprehending cell wall architecture and wine quality.

Purpose of the Study:

  • To isolate and characterize RG-II from red wine polysaccharides.
  • To compare the structural features of wine RG-II with those from primary cell walls.

Main Methods:

  • Anion-exchange chromatography for initial isolation.
  • Concanavalin A affinity and size-exclusion chromatography for purification.
  • Gas-phase electron ionization mass spectrometry (GC-EIMS) for linkage analysis.

Main Results:

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  • Four RG-II fractions were isolated, all containing diagnostic monosaccharides like apiose and aceric acid.
  • Two RG-II fractions formed dimers via borate di-ester cross-links, suggesting a role in structural integrity.
  • Structural variations were observed in backbone length, methyl-esterification, and borate di-ester presence.

Conclusions:

  • Wine RG-II shares conserved major structural features with primary cell wall RG-II.
  • Borate di-esters play a role in RG-II dimerization in wine.
  • Structural heterogeneity exists within wine RG-II populations.