Nucleotide sequence of a plasmid pCL2.1 from Lactococcus lactis ssp. lactis ML8
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1Department of Food Science and Technology, Seoul National University, 441-744 Suwon, Korea.
Plasmid
|November 1, 1995
Abstract:
The nucleotide sequence of a small cryptic plasmid, pCL2.1, from Lactococcus lactis ssp. lactis ML8 was determined. Sequence analysis of the pCL2.1 revealed that it contained 2112 bp, 33.9% GC, and two open reading frames that encoded polypeptides of 26 and 14 kDa. In vitro transcription-translation of the pCL2.1 confirmed the existence of two polypeptides. Based on sequence homology, it is deduced that the ORF2 product functions in plasmid replication by a rolling circle mechanism.
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