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Updated: Aug 3, 2026
Osteoclast Derivation from Mouse Bone Marrow
Published on: November 6, 2014
Stimulation of osteoclast-like cell formation by Pasteurella multocida toxin from hemopoietic progenitor cells in
1Groupe de Recherche sur les Maladies du Porc (GREMIP), Faculty of Veterinary Medecine, University of Montreal, St-Hyacinthe, Québec.
Abstract:
The effects of purified Pasteurella multocida toxin (PMT) on osteoclast formation from hemopoietic progenitor cells were studied using an in vitro system. Mononuclear adherent mouse bone marrow cells were cultured for 7 or 14 days in the presence of PMT, or 1,25-dihydroxy-vitamin D3, or both. Mononuclear osteoclast-like cells, which are postmitotic osteoclast precursor cells, were identified as tartrate-resistant acid phosphatase (TRAP)-positive mononuclear cells possessing calcitonin receptors. Multinucleated osteoclast-like cells were TRAP-positive multinuclear cells with calcitonin receptors. The results demonstrate that, as does 1,25(OH)2D3, Pasteurella multocida toxin stimulates proliferation of adherent bone marrow mononuclear cells (progenitor cells), and their differentiation into postmitotic mononuclear osteoclast precursor cells. It also causes fusion of the latter into multinuclear osteoclasts; however, the number of osteoclasts obtained with PMT is smaller than with 1,25-dihydroxy-vitamin D3.
Insights
Pasteurella multocida toxin (PMT) stimulates osteoclast formation from progenitor cells, similar to vitamin D. However, PMT induces fewer osteoclasts compared to vitamin D in this in vitro study.
Area of Science:
- Cell Biology
- Immunology
- Microbiology
Background:
- Osteoclasts are crucial for bone remodeling.
- Hemopoietic progenitor cells differentiate into osteoclasts.
- Pasteurella multocida toxin (PMT) is a virulence factor.
Purpose of the Study:
- To investigate the effects of PMT on osteoclastogenesis.
- To compare PMT's osteoclastogenic activity with 1,25-dihydroxy-vitamin D3 (1,25(OH)2D3).
Main Methods:
- In vitro culture of mouse bone marrow mononuclear cells.
- Treatment with PMT, 1,25(OH)2D3, or both.
- Identification of osteoclast precursors and multinucleated osteoclasts via TRAP staining and calcitonin receptors.
Main Results:
- PMT stimulates proliferation of progenitor cells and their differentiation into osteoclast precursors.
- PMT induces fusion of precursors into multinucleated osteoclasts.
- PMT elicits a lower number of osteoclasts compared to 1,25(OH)2D3.
Conclusions:
- PMT possesses osteoclast-stimulating properties.
- PMT influences early stages of osteoclast formation.
- PMT's effect on osteoclastogenesis is less potent than 1,25(OH)2D3.
