Related Experiment Video
Updated: Aug 14, 2026

Ferric Chloride-induced Murine Thrombosis Models
Published on: September 5, 2016
Cisplatin-induced changes in biological activity of blood platelets: thiol-related mechanisms
1Department of General Biochemistry, University of Banacha, Lódź, Poland.
Abstract:
The effects of cisplatin (cis-diamminedichloroplatinum (II), CDDP) and transplatin (trans-diamminedichloroplatinum(II), TDDP), and their complexes with glutathione (GS-Pt) in vitro on oxygen free radical generation O2-.), lipid peroxidation and ADP-induced aggregation in pig blood platelets were studied. Incubation of pig blood platelets with cisplatin or transplatin caused a loss of both protein -SH and the thiol groups of GSH. In pig blood platelets exposed to cisplatin or transplatin (20 microM) the GS-Pt complex was formed as a major metabolite. The formation of GS-Pt complexes in platelet cytosol was time dependent and the intracellular content of this complex reached a maximal level after 24 h. GS-Pt complexes were found to induce platelet lipid peroxidation, measured as thiobarbituric acid reactive substance level, and O2-. generation, and it was more active than cisplatin or transplatin alone. Cisplatin and its GS-Pt complex had an inhibitory effect on ADP-induced platelet aggregation. These results showed that GS-Pt complexes affect platelet metabolism and function. It seems that glutathione-associated metabolism of platinum compounds plays an important role in the cytotoxicity and biological activity of these drugs.
More Related Videos
05:49Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
Published on: November 29, 2024
04:37Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
Published on: May 23, 2025
Related Concept Videos
Drugs that Destabilize Microtubules
Intracellular Signaling Affects Focal Adhesions
Some...
Anticoagulant Drugs: Low-Molecular-Weight Heparins
Structure and Function of Platelets
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Formation of the Platelet Plug
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...