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An Orthotopic Murine Model of Human Prostate Cancer Metastasis

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Clinical study on estramustine binding protein (EMBP) in human prostate

H Shiina¹, M Igawa, T Ishibe

¹Department of Urology, Shimane Medical University, Izumo, Japan.

|September 1, 1996

Summary

Estramustine binding protein (EMBP) levels in prostate tissue correlate with androgen dependency. The EMBP/DHT ratio may predict prostate cancer progression, distinguishing it from prostate-specific antigen (PSA).

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Area of Science:

Urology
Oncology
Biochemistry

Background:

Estramustine binding protein (EMBP) is implicated in prostate tissue.
Androgen-dependent markers like dihydrotestosterone (DHT) and prostate-specific antigen (PSA) are crucial in prostate health.
Understanding EMBP's role is key to characterizing prostate conditions.

Purpose of the Study:

To investigate the characteristics of estramustine binding protein (EMBP) in human prostate tissues.
To compare EMBP concentrations with established androgen-dependent markers (DHT, PSA, prostatic acid phosphatase, zinc).
To evaluate the potential of EMBP as a biomarker for prostate cancer progression.

Main Methods:

Radioimmunoassay (RIA) was used to measure tissue EMBP concentration in control, benign prostatic hypertrophy (BPH), untreated prostate carcinoma (PC), and hormone-refractory PC (hr-PC) samples.
EMBP concentrations were correlated with DHT, PSA, prostatic acid phosphatase (PAP), and zinc levels.
The EMBP/DHT ratio was analyzed in relation to disease differentiation and progression.

Main Results:

EMBP concentration correlated with DHT and PSA in controls and BPH, but not in untreated PC.
EMBP levels in BPH responded to fluoxymesterone and estramustine phosphate administration.
The EMBP/DHT ratio was significantly higher in advanced PC (moderately/poorly differentiated, hr-PC) and associated with lower progression-free probability in untreated PC.

Conclusions:

EMBP in BPH and well-differentiated PC exhibits androgen dependency, unlike in advanced or hormone-refractory prostate cancer.
EMBP appears to be a distinct protein from PAP and PSA.
The tissue EMBP/DHT ratio shows potential as a predictive marker for prostate cancer disease progression.