Sputum bacteriology and its antibiotic susceptibilities in Turkish cystic fibrosis patients
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1Department of Pediatrics, Hacettepe University Faculty of Medicine, Ankara.
Insights
This study identified common respiratory pathogens in Turkish children with cystic fibrosis (CF). Antibiotic susceptibility testing revealed effective treatments for key bacteria like Pseudomonas aeruginosa and Staphylococcus aureus.
Area of Science:
- Microbiology
- Pediatrics
- Infectious Diseases
Background:
- Cystic Fibrosis (CF) patients are prone to chronic respiratory infections.
- Understanding local pathogen prevalence and antibiotic resistance is crucial for effective CF management.
Purpose of the Study:
- To determine the common lower respiratory tract pathogens in Turkish CF children.
- To evaluate the in-vitro antibiotic susceptibilities of these isolated pathogens.
Main Methods:
- Analysis of 383 sputum cultures from 45 Turkish CF children over 25 months.
- Microbiological isolation and identification of pathogens during symptomatic and control periods.
- In-vitro antibiotic susceptibility testing of common isolates.
Main Results:
- Microorganisms were isolated in 65.8% of cultures (82% symptomatic, 53% control).
- Pseudomonas aeruginosa and Staphylococcus aureus were the most prevalent pathogens.
- High in-vitro susceptibility was observed for P. aeruginosa to amikacin, ciprofloxacin, and ceftazidime; S. aureus to cefuroxime, ceftriaxone, amikacin, cephalothin, chloramphenicol, and erythromycin.
Conclusions:
- The identified pathogens and their antibiotic susceptibilities in Turkish CF children align with existing literature.
- This data supports targeted antimicrobial therapy for respiratory infections in this population.
Abstract:
To identify lower respiratory tract pathogens and their in-vitro antibiotic susceptibilities in Turkish cystic fibrosis (CF) patients, a total of 383 sputum cultures were evaluated from 45 CF children in 168 symptomatic and 215 control periods over 25 months. Microorganisms were isolated in 252 of the cultures. The isolation rate was 82 percent for symptomatic periods and 53 percent for control periods. The most common microorganism was P. aeruginosa in the symptomatic period and S. aureus in the control period. Other microbiological species included E. coli, H. influenzae, K. pneumoniae, S. epidermidis, beta-hemolytic streptococcus, H. parainfluenzae, K. oxytoca, E. aerogenes and E. aglomerans. P. cepacia was not found. In 20 cultures more than one microorganism was isolated at the same time. In in-vitro conditions, high susceptibility rates were detected to amikacin, ciprofloxacin and ceftazidim for P. aeruginosa; cefuroxime, ceftriaxone, amikacin, cephalothin, chloramphenicol and erythromycin for S. aureus; amikacin and ceftriaxone for E. coli; ampicillin-sulbactam, amoxicillin-clavulanate, cefuroxime, ceftazidime, ceftriaxone and aztreonam for H. influeanzae; and aztreonam and amikacin for K. pneumoniae. Lower respiratory tract pathogens and their antibiotic susceptibilities in Turkish CF children were not significantly different from those indicated previously in the literature.