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Repetitive element sequence based polymerase chain reaction for typing of Brucella strains
E Tcherneva1, N Rijpens, C Naydensky
1Central Veterinary Research Institute, Sofia, Bulgaria.
Veterinary Microbiology
|July 1, 1996
Abstract:
A collection of 40 Brucella strains, including 19 clinical B.canis isolates, was analyzed using ERIC-PCR and REP-PCR. PCR amplification using primers REP 1R-I and REP 2-I provided distinct patterns for Brucella spp. All Brucella strains could be discriminated at least to the species level. ERIC-PCR, employing ERIC 1R and ERIC 2 as primers was less discriminative for Brucella, only allowing a discrimination to the genus level with occasional discrimination between individual strains. It can be concluded that rep PCR is a promising fingerprinting method for the evaluation of an outbreak.