Enzyme support systems for biosensor applications based on gold-coated nylon meshes

Area of Science:

• Biochemistry
• Electrochemistry
• Biosensor Technology

Background:

• Enzyme immobilization is crucial for biosensor stability and reusability.
• Developing highly permeable supports enhances biosensor performance.
• Acetylcholinesterase (AChE) is a key enzyme for detecting pesticides.

Purpose of the Study:

• To develop a novel enzyme immobilization protocol using a permeable support.
• To create an acetylcholinesterase (AChE) based biosensor for pesticide detection.
• To characterize the immobilization support and the assembled biosensor.

Main Methods:

• Enzyme immobilization onto a gold-coated nylon mesh using cystamine self-assembled monolayers.
• Characterization of the support via optical microscopy and electrochemical permeability measurements.
• Assembly of the biosensor with AChE-modified mesh over a glassy carbon electrode.
• Monitoring of 4-aminophenol production from 4-aminophenylacetate substrate via electrochemical oxidation.

Main Results:

• Successful immobilization of AChE on a permeable gold-coated nylon mesh.
• Demonstration of the biosensor's response to the substrate 4-aminophenylacetate.
• Validation of the biosensor for detecting organophosphorus and carbamate pesticides through AChE inhibition.

Conclusions:

• The novel immobilization protocol provides a robust and efficient method for biosensor development.
• The developed AChE biosensor is effective for the sensitive determination of pesticides.
• This approach offers a versatile platform for various enzyme-based sensing applications.