Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TSH receptor in endocrine autoimmunity

E Otto1, G Förster, K Kuhlemann

  • 1Department of Medicine III, Johannes Gutenberg, University Hospital, Mainz, Germany.

Clinical and Experimental Rheumatology
|May 1, 1996
PubMed
Summary

Researchers found partial human thyrotropin receptor (hTSHR) transcripts in orbital tissues, but not functional expression. This suggests a non-functional part of hTSHR may contribute to autoimmune eye disease, though its role as a common antigen remains unclear.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes of self-trial without catheter pathway following elective urological surgery.

Annals of the Royal College of Surgeons of England·2026
Same author

The benefits and societal importance of pig welfare in slaughterhouses a narrative review.

Meat science·2025
Same author

The Magnetic Search Coil (MSC) on the TRACERS Mission.

Space science reviews·2025
Same author

Low latency optical-based mode tracking with machine learning deployed on FPGAs on a tokamak.

The Review of scientific instruments·2024
Same author

Tangential extreme ultraviolet and soft x-ray diagnostic system for time-resolved temperature measurement on the High Beta Tokamak-Extended Pulse.

The Review of scientific instruments·2023
Same author

Assistance dogs help reduce mental health symptoms among Australian Defence Force veterans and emergency services personnel: A pilot study.

Psychiatry research·2023

Area of Science:

  • Endocrinology
  • Immunology
  • Ophthalmology

Background:

  • The human thyrotropin receptor (hTSHR) is implicated in endocrine autoimmunity.
  • Previous studies conflict on hTSHR transcript presence in extrathyroidal tissues of autoimmune thyroid disease (ATD) patients.

Purpose of the Study:

  • Investigate hTSHR expression in orbital adipose/connective and muscle tissue.
  • Examine orbital fibroblasts and myoblasts for functional hTSHR in thyroid and eye disease patients.

Main Methods:

  • Reverse transcription and PCR amplification of hTSHR mRNA.
  • Tetrazolium assay to measure TSH-mediated metabolic activity in cultured cells.
  • Analysis of retrobulbar and retro-ocular tissues.

Main Results:

Related Experiment Videos

  • Transcripts for transmembrane and intracellular domains of hTSHR were detected in orbital tissues and cell cultures.
  • Extracellular domain amplification was unsuccessful.
  • No functional stimulation of fibroblasts or myoblasts by TSH was observed.
  • hTSHR transcripts were also found in healthy individuals' retro-ocular tissue.

Conclusions:

  • hTSHR is not expressed in a functional form in orbital tissue.
  • A non-functional, antigenic portion of hTSHR might play a role in autoimmune eye disease pathogenesis.
  • The link between hTSHR and a common thyroid-orbit antigen requires further clarification.