Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HPr: a model protein

J W Anderson1

  • 1Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Canada.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

Histidine-containing protein (HPr) is key to bacterial sugar uptake. Recent studies reveal how its active site residues influence phosphotransferase activity, phosphohydrolysis, and His 15

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Case of a Peculiar Form of Asthma.

Glasgow medical journal·2018
Same author

Meningocele.

Edinburgh medical journal·2018
Same author

Light-dependent reduction of dehydroascorbate and uptake of exogenous ascorbate by spinach chloroplasts.

Planta·2013
Same author

Oxygen evolution by a reconstituted spinach chloroplast system in the presence ofL-glutamine and 2-oxoglutarate.

Planta·2013
Same author

Ammonia assimilation and oxygen evolution by a reconstituted chloroplast system in the presence of 2-oxoglutarate and glutamate.

Planta·2013
Same author

Chitin-glucan fiber effects on oxidized low-density lipoprotein: a randomized controlled trial.

European journal of clinical nutrition·2012
Same journal

Reply to the discussion of "Efficacy of lactoferrin supplementation in pediatric infections: a systematic review and metanalysis".

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

Discussion of "Efficacy of lactoferrin supplementation in pediatric infections: a systematic review and meta-analysis".

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

Does the beta subunit of mitochondrial trifunctional protein modulate monolysocardiolipin acylation to cardiolipin?

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

Distinct effects of disease-associated mutations in the proximal C-linker on negatively cooperative binding of cyclic nucleotides to the human HCN4 channel.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

PPP2R2B targets the JAK2-STAT3 signaling pathway to regulate ferroptosis in breast cancer cells.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
Same journal

Suppression of CBP/p300 induces growth inhibition and autophagy of hepatocellular carcinoma cells through regulation of EPHA2 by its super-enhancer acetylation.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2026
See all related articles

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Microbiology

Background:

  • The phosphoenolpyruvate: sugar phosphotransferase system (PTS) is crucial for bacterial carbohydrate metabolism.
  • Histidine-containing protein (HPr) is a central protein within the bacterial PTS.
  • Understanding HPr's structure-function relationship is vital for elucidating PTS mechanisms.

Purpose of the Study:

  • To review recent structure-function studies on the active center of Histidine-containing protein (HPr).
  • To elucidate the roles of active site residues in phosphotransferase activity and phosphohydrolysis.
  • To examine the contribution of residues to the pKa of the phosphorylated active-site residue, His 15.

Main Methods:

  • Review of recent structure-function studies.

Related Experiment Videos

  • Analysis of active center residues.
  • Investigation of phosphotransferase and phosphohydrolysis properties.

    • Main Results:

    • Identified key roles of active center residues in HPr's phosphotransferase function.
    • Characterized residues contributing to HPr's phosphohydrolysis capabilities.
    • Detailed the influence of specific residues on the pKa of the active-site histidine (His 15).

    Conclusions:

    • Active site residues critically determine HPr's enzymatic properties within the PTS.
    • HPr's phosphohydrolysis and pKa modulation are directly linked to its active center composition.
    • HPr serves as a valuable model protein for broader biochemical and structural studies beyond its PTS role.