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Phorbol ester or cAMP enhance thyrotropin-releasing hormone mRNA in primary cultures of hypothalamic cells
R M Uribe1, L Pérez-Martínez, M de Lourdes Covarrubias
1Departamento de Genética y Fisiología Molecular, Universidad Nacional Autónoma de México.
Abstract:
Thyrotropin releasing hormone (TRH) biosynthesis is subject to a multifactorial control. TRH mRNA levels are negatively regulated by thyroid hormones in the paraventricular hypothalamic nucleus, and positively in cold exposure or suckling. Effect of second messenger pathways stimulation, a known response to membrane receptors, was studied in vitro; cultures of rat embryonic hypothalami (18 day gestation) were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA, 100 nM) or dibutiryl cAMP (dBcAMP, 1 mM) for various times. Levels of TRH mRNA were raised after the first hour of dBcAMP or 2 h of TPA treatment and were still increased at 24 h. These results suggest a neural regulation of TRH biosynthesis.