Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Paraprotein interference causing pseudohyperphosphataemia: evaluation of an improved methodology

D J Savory1, C J Pearce

  • 1Department of Clinical Biochemistry, Ipswich Hospital NHS Trust, UK.

Annals of Clinical Biochemistry
|September 1, 1995
PubMed
Summary

Direct acid/molybdate assays for serum inorganic phosphorus can be affected by paraproteins. A modified reagent eliminated interference, preventing pseudohyperphosphataemia in paraproteinaemic patients.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A non-randomised pilot study of the Solutions for Medication Adherence Problems (S-MAP) intervention in community pharmacies to support older adults adhere to multiple medications.

Pilot and feasibility studies·2021
Same author

Recent developments in equine dentistry.

New Zealand veterinary journal·2020
Same author

Fungal Planet description sheets: 785-867.

Persoonia·2019
Same author

Endoscopic-assisted local intralesional curettage and excision of intraosseous lesions in the talus.

Annals of the Royal College of Surgeons of England·2017
Same author

'New to me': changing patient understanding of psoriasis and identifying mechanisms of change. The Pso Well<sup>®</sup> patient materials mixed-methods feasibility study.

The British journal of dermatology·2017
Same author

Verticillin A Inhibits Leiomyosarcoma and Malignant Peripheral Nerve Sheath Tumor Growth via Induction of Apoptosis.

Clinical & experimental pharmacology·2017

Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Hematology

Background:

  • Direct acid/molybdate assays for serum inorganic phosphorus are susceptible to interference from paraproteins.
  • Paraproteins can lead to inaccurate pseudohyperphosphataemia results in patient samples.

Purpose of the Study:

  • To evaluate a detergent-modified reagent for measuring serum inorganic phosphorus.
  • To assess the interference of paraproteins on inorganic phosphorus measurements.
  • To validate a modified assay free from paraprotein interference.

Main Methods:

  • Serum samples from 45 paraproteinaemic patients were analyzed.
  • Inorganic phosphorus was measured using a standard manufacturer's kit reagent.
  • Measurements were repeated with a detergent-modified reagent and after trichloroacetic acid protein precipitation.

Related Experiment Videos

Main Results:

  • The original reagent showed pseudohyperphosphataemia in 27% (12/45) of samples.
  • The detergent-modified reagent eliminated interference from paraproteins.
  • No significant difference was observed between serum and protein-free supernatant results with the modified reagent.

Conclusions:

  • The detergent-modified reagent effectively removes paraprotein interference in serum inorganic phosphorus assays.
  • This modified assay provides accurate inorganic phosphorus measurements in paraproteinaemic patients.
  • The modified reagent is a reliable alternative for clinical chemistry laboratories handling such samples.