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Mutagenic activity of various dentine bonding agents
H Schweikl1, G Schmalz, C Göttke
1Department of Operative Dentistry and Periodontology, University of Regensburg, Germany.
Biomaterials
|July 1, 1996
Summary
New dental bonding agents were tested for mutagenicity using the Ames test. One agent containing glutaraldehyde showed mutagenic effects, prompting further investigation into potential risks.
Area of Science:
- Dental Materials Science
- Toxicology
- Genetics
Background:
- Dentine bonding systems are crucial in modern restorative dentistry.
- Assessing the safety of new dental materials is essential.
- The potential mutagenicity of novel bonding agents requires thorough evaluation.
Purpose of the Study:
- To characterize the in vitro mutagenicity of components from three new-generation dentine bonding systems.
- To identify specific components or formulations that may pose a mutagenic risk.
Main Methods:
- Utilized the Ames test, an in vitro gene mutation assay, with four Salmonella strains (TA97a, TA98, TA100, TA102).
- Tested eight components from Scotchbond Multi Purpose, Prisma Universal Bond 3, and C&B Metabond.
- Materials were eluted in dimethyl sulfoxide and physiological saline before standard plate incorporation assay.
Main Results:
- Scotchbond Multi Purpose primer/adhesive, Prisma Universal Bond 3 primer, and C&B Metabond base/powder/activator showed no mutagenicity.
- Prisma Universal Bond 3 adhesive, containing glutaraldehyde, demonstrated significant mutagenicity in Salmonella strain TA102.
- C&B Metabond catalyst also exhibited mutagenic effects, particularly in strain TA97a when eluted in saline.
Conclusions:
- The Ames test identified potential mutagenicity in specific components of new dentine bonding systems.
- Glutaraldehyde-containing Prisma Universal Bond 3 adhesive and C&B Metabond catalyst warrant further safety assessment.
- Results suggest the need for further investigation using mammalian cell assays to evaluate risks in higher organisms.