Metabolic, endocrine and haematological responses to intravenous E. coli endotoxin administration in 1-week-old...

Responses to i.v. injected E. coli endotoxin (E), followed by saline infusion, as compared with saline infusion alone, were studied for 24 h in 1-week-old calves. After administration of E, respiratory rate (RR), heart rate (HR), rectal temperature (RT), serum iron, insulin, (I), cortisol and tumor necrosis factor-alpha, transiently, and urea, continuously, increased. Isoleucine and leucine became elevated at 24 h, whereas white-blood-cell number, free fatty acids (FFA) and triglycerides (TG) increased after an initial fall. After administration of E, packed-cell volume, erythrocyte number, haemoglobin, glucose (G), cholesterol, phospholipids (PL), lysine, arginine, proline, citrulline, calcium (Ca), inorganic phosphorus, insulin-like growth factor I (IGF-I) and 3,5,3'-triiodothyronine (T3) concentrations and alkaline phosphatase (AP) and gamma-glutamyl transferase (gamma GT) activities increased significantly while growth hormone decreased non-significantly. When saline was infused alone, G, TG, PL, Ca, AP, gamma GT, I, IGF-I and T3 decreased, while FFA, urea and sodium increased, but, changes of G, urea, AP, IGF-I and T3 were less marked than after injection of E. Potassium, total protein and albumin concentrations, and glutamyl dehydrogenase and glutamate oxalacetate transaminase activities were not significantly affected by either treatment. In conclusion, metabolic and endocrine changes during saline infusion alone were typical for food withdrawal. Changes of variables after administration of E were transient, biphasic or sustained, thus expressing complex interactions between metabolic parameters, endocrine factors and cytokines.