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Related Experiment Videos

Ca2+ waves in lung capillary endothelium

X Ying1, Y Minamiya, C Fu

  • 1Department of Medicine, College of Physicians & Surgeons, Columbia University, St. Luke's-Roosevelt Hospital Center, New York, NY 10019, USA.

Circulation Research
|October 1, 1996
PubMed
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Lung capillary endothelial cells exhibit intercellular calcium (Ca2+) waves. These waves, originating from pacemaker cells, regulate Ca2+ levels in lung microvasculature under resting and stimulated conditions.

Area of Science:

  • Physiology
  • Cell Biology
  • Pulmonary Medicine

Background:

  • Cytosolic Ca2+ is crucial for organ function.
  • Lung microvascular Ca2+ regulation is poorly understood due to limited in situ quantification.

Purpose of the Study:

  • To quantify endothelial intracellular Ca2+ ([Ca2+]i) in rat lung capillaries.
  • To investigate the mechanisms and characteristics of intercellular Ca2+ waves in lung capillaries.

Main Methods:

  • Fura 2 imaging in isolated blood-perfused rat lungs.
  • Microscopic observation of venular capillaries.
  • Fast Fourier analysis of Ca2+ oscillations.
  • Inhibition studies using thapsigargin and heptanol.

Main Results:

Related Experiment Videos

  • First direct quantification of endothelial [Ca2+]i in lung capillaries.
  • Identified spontaneous intercellular Ca2+ waves originating from pacemaker cells.
  • Waves propagated via gap junctional communication and involved endosomal Ca2+ release.
  • Histamine enhanced these waves, contributing to coordinated [Ca2+]i increase.

Conclusions:

  • Discovered a novel mechanism of [Ca2+]i regulation in lung capillaries.
  • Pacemaker-induced Ca2+ waves play a role in both resting and stimulated lung microvascular function.
  • Intercellular Ca2+ waves determine [Ca2+]i profiles in capillary segments.