Some properties of a cyclomaltodextrin-glucanotransferase from Bacillus circulans DF 9 R type

¹Instituto de Investigaciones Bioquímicas Luis F. Leloir Fundación Campomar, Buenos Aires, Argentina.

Cellular and Molecular Biology (Noisy-Le-Grand, France)

|July 1, 1996

Abstract:

The cyclomaltodextrin-glucanotransferase (EC2.4.1.19, CGTase) which was purified to homogeneity from Bacillus circulans strain DF 9, R type, showed a pI of 5.3 determined by disc-isoelectric focusing, a Mw of 78 kDa estimated by SDS-PAGE with a range of pH of optimal enzymatic activity rather large (4.5-7.5). The thermal stability of the enzyme at 55 degrees C was increased 4-5 times when calcium ion (10 to 100 mM) or alpha-cyclodextrins (10 mM) were added to the preincubation mixtures. The alpha: beta: gamma ratio determined by HPLC was about 1:0.9:0.4 and the maximal conversion to cyclodextrins with 5% soluble starch was about 36%.

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