Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-specific DNA modification in Acetobacter xylinum

E A Petroni1, S N Bocca, L Ielpi

  • 1Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|July 1, 1996
PubMed
Summary

Acetobacter xylinum B42 harbors two cryptic plasmids, pAX1 and pAX2. These bacteria possess a modification system protecting specific DNA sequences from restriction enzymes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of interspecies interactions in dual-species biofilms developed in vitro by uropathogens isolated from polymicrobial urinary catheter-associated bacteriuria.

Biofouling·2016
Same author

Binding of the substrate UDP-glucuronic acid induces conformational changes in the xanthan gum glucuronosyltransferase.

Protein engineering, design & selection : PEDS·2016
Same author

Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from Xanthomonas campestris.

Protein expression and purification·2014
Same author

Identification, genetic and biochemical analysis of genes involved in synthesis of sugar nucleotide precursors of xanthan gum.

Journal of general microbiology·2010
Same author

[Vaccine production in Argentina: a decision that cannot be postponed].

Medicina·2003
Same author

Occurrence of a putative SCF ubiquitin ligase complex in Drosophila.

Biochemical and biophysical research communications·2001

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Acetobacter xylinum B42 is known for cellulose production.
  • Plasmids are extrachromosomal DNA elements found in bacteria.
  • Understanding plasmid content is crucial for bacterial genetics.

Purpose of the Study:

  • To identify and characterize cryptic plasmids in Acetobacter xylinum B42.
  • To investigate potential DNA modification systems in this bacterium.

Main Methods:

  • Plasmid isolation and characterization (size determination, restriction mapping).
  • Enzyme restriction analysis of plasmids and chromosomal DNA.
  • Southern blot and hybridization techniques.

Main Results:

Related Experiment Videos

  • Two cryptic plasmids, pAX1 (50 kb) and pAX2 (105 kb), were identified in Acetobacter xylinum B42.
  • pAX1 and pAX2, as well as other plasmids in derivative strain PEA-1, exhibited protection against EcoRI and ApoI restriction enzymes.
  • This protection was also observed in chromosomal DNA, suggesting a widespread modification system.
  • The modification system appears to recognize the tetranucleotide sequence 5'-AATT.

Conclusions:

  • Acetobacter xylinum B42 possesses a DNA modification system.
  • This system protects the 5'-AATT sequence from cleavage by specific restriction enzymes.
  • The presence of protected sites on plasmids and chromosomal DNA has implications for genetic manipulation and understanding bacterial defense mechanisms.