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Published on: December 21, 2010
Theory of gel electrophoresis of DNA: orientational effects
1Institute of Petrochemical Synthesis, Russian Academy of Science, Moscow, Russia. tips@tips.rc.ac.ru
Abstract:
Some aspects of the reptation model of gel electrophoresis of DNA, with emphasis on the orientational effects, are considered. A master equation for the reptational dynamics with the end boundary conditions is proposed. Based on this equation, the orientation and dynamics of the persistent-chain are investigated. In particular, the role of trap conformations for this chain, which essentially reduce the chain mobility, was analyzed. A transient orientation of the chain during pulsed field gel electrophoresis is also discussed.
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