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Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in
J J Schwinefus1, S C Wang, R W Hammond
1University of Michigan, Department of Chemistry, Ann Arbor 48109-1055, USA.
Electrophoresis
|June 1, 1996
Abstract:
Fluorescence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random entanglement of DNA in the outer portions. HEC concentrations also appear to play a role in aggregate formation. DNA aggregates take longer to assemble in higher HEC concentration solutions and assume a less compact form than those formed at lower HEC concentrations.