Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A basic scientist's odyssey in nutrition.

European journal of clinical nutrition·2018

Same author

Circulating Levels of the Adipokines Monocyte Chemotactic Protein-4 (MCP-4), Macrophage Inflammatory Protein-1β (MIP-1β), and Eotaxin-3 in Severe Obesity and Following Bariatric Surgery.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2016

Same author

Functional body composition and related aspects in research on obesity and cachexia: report on the 12th Stock Conference held on 6 and 7 September 2013 in Hamburg, Germany.

Obesity reviews : an official journal of the International Association for the Study of Obesity·2014

Same author

Semaphorin 3C is a novel adipokine linked to extracellular matrix composition.

Diabetologia·2013

Same author

1,25-Dihydroxyvitamin D3 inhibits the cytokine-induced secretion of MCP-1 and reduces monocyte recruitment by human preadipocytes.

International journal of obesity (2005)·2012

Same author

11-Hydroxy-β-steroid dehydrogenase gene expression in canine adipose tissue and adipocytes: stimulation by lipopolysaccharide and tumor necrosis factor α.

Domestic animal endocrinology·2011

Same journal

Nutritional and dietary drivers in the pathogenesis of acute appendicitis: the nutrition-microbiome-genetic axis.

The Proceedings of the Nutrition Society·2026

Same journal

Redistributing power in food systems: How community-led responses move from crisis buffering to systems transformation.

The Proceedings of the Nutrition Society·2026

Same journal

Using positive social influences to increase children's vegetable intake.

The Proceedings of the Nutrition Society·2026

Same journal

Combining sequence-based approaches with anaerobic microbiology and modelling to understand gut microbial communities.

The Proceedings of the Nutrition Society·2026

Same journal

Misled or misfed: Nutrition claims targeting active females on social media, is there research evidence to support them?

The Proceedings of the Nutrition Society·2026

Same journal

Whole-body metabolic modelling and its prospects in precision nutrition.

The Proceedings of the Nutrition Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 26, 2025

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Northern blotting

¹Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen.

The Proceedings of the Nutrition Society

|March 1, 1996

Summary

This summary is machine-generated.

Northern blotting measures gene expression via mRNA. Non-radioactive methods using oligonucleotide probes and chemiluminescence simplify this crucial technique for nutritional studies.

More Related Videos

Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Published on: August 21, 2007

Related Experiment Videos

Last Updated: Dec 26, 2025

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Published on: August 21, 2007

Area of Science:

Molecular Biology
Gene Expression Analysis

Background:

Northern blotting is a primary technique for assessing gene expression by measuring mRNA levels.
It is essential for determining tissue specificity and regulatory factors of gene expression.

Purpose of the Study:

To introduce a simplified and rapid Northern blotting protocol.
To enhance the accessibility of Northern blotting for nutritional studies.

Main Methods:

Utilized anti-sense oligonucleotides as hybridization probes.
Employed chemiluminescence-based detection for signal visualization.
Compared with traditional radioactive probes (e.g., 32P-labeled cDNA).

Main Results:

The combination of oligonucleotide probes and chemiluminescence offers a rapid and simplified Northern blotting approach.
This method increases the accessibility of Northern blotting for various research applications, including nutritional studies.

Conclusions:

Non-radioactive detection methods, particularly with oligonucleotide probes and chemiluminescence, represent a significant advancement in Northern blotting.
This streamlined technique broadens the utility of gene expression analysis in diverse research fields.