Related Experiment Video
Updated: Dec 26, 2025
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
Published on: August 21, 2014
Northern blotting
1Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen.
Abstract:
Northern blotting is the main method for examining the expression of genes through measurement of an mRNA. Tissue specificity and the factors which regulate expression can both be determined by Northern blotting. A cDNA radioactively labelled with 32P is the most-commonly-used hybridization probe. Convenient, non-radioactive detection protocols are, however, increasingly available. A combination of anti-sense oligonucleotides as probes, together with chemiluminescence-based detection provides a rapid and simplified approach to Northern blotting, increasing the accessibility of this important procedure for nutritional studies.
Related Concept Videos
02:57Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
01:15Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.